HistologyHSI-GB | Hyperspectral Histological Images for Diagnosis of Human Glioblastoma

DOI: 10.7937/z1k6-vd17 | Data Citation Required | Image Collection

Summary

From: Histological Hyperspectral Glioblastoma Dataset (HistologyHSI-GB) - https://www.nature.com/articles/s41597-024-03510-x

Hyperspectral imaging technology combines the main features of two existing technologies: conventional imaging and spectroscopy. Thus, hyperspectral cameras make it possible to analyze, at the same time and in a non-contact way, the morphological features and chemical composition of the objects captured. The information provided by hyperspectral imaging can be used to detect patterns, cells, or biomarkers to identify diseases. There are different alternatives for processing them and there is a lack of publicly available datasets of medical hyperspectral images. To the best of our knowledge, this is the first open access dataset containing histological hyperspectral images of glioblastoma brain tumors, which can be set as a benchmark for researchers to compare their approaches.

This dataset includes 13 subjects.  Each subject has a single histological slide with multiple hyperspectral images captured from each slide where deemed relevant by the pathologists (this number varies for each slide). The database is composed of 469 annotated hyperspectral images from 13 histological slides (482 total images), having a spatial dimension of 800 × 1004 pixels and a spectral dimension of 826 spectral channels. The format of the hyperspectral images is ENVI, the standard format for the storage of hyperspectral images. The ENVI format consists of a flat-binary raster file which may or may not have a file extension, accompanied by an ASCII header file (denoted as *.hdr). The data are stored in band-interleaved-by-line format. In addition, dark and white references were captured to perform a calibration of the raw image, which is a standard procedure in hyperspectral image processing.

The slides were stained with hematoxylin and eosin and captured using a custom hyperspectral microscopic system at 20× magnification. The ground-truth annotation for this dataset is the diagnosis of the slides (tumor or not tumor) performed by skilled histopathologists after the visual examination of the stained slides, according to the World Health Organization classification of tumors of the nervous system. As far as we are concerned, there are no commercial hyperspectral whole slide scanners. Also, the availability of hyperspectral microscopes is still limited in the market.  

The microscope is an Olympus BX-53 (Olympus, Tokyo, Japan). The hyperspectral camera is a Hyperspec® VNIR A-Series from HeadWall Photonics (Fitchburg, MA, USA), which is based on an imaging spectrometer coupled to a charge-coupled device sensor, the Adimec-1000m (Adimec, Eindhoven, Netherlands). This hyperspectral system works in the visual and near-infrared spectral range from 400 to 1000 nm with a spectral resolution of 2.8 nm, sampling 826 spectral channels, and 1004 spatial pixels. The push-broom camera performs a spatial scanning to acquire a hyperspectral cube with a mechanical stage (SCAN, Märzhäuser, Wetzlar, Germany) attached to the microscope, which provides an accurate movement of the slides. The objective lenses are from the LMPLFLN family (Olympus, Tokyo, Japan), optimized for infrared observations.

More information about the dataset can be found in this manuscript.

Data Access

Version 1: Updated 2024/05/24

Additional Resources for this Dataset

The following external resources have been made available by the data submitters.  These are not hosted or supported by TCIA, but may be useful to researchers utilizing this collection.

• A tutorial on how to read and display the hyperspectral data using Python or MATLAB is available at https://github.com/HIRIS-Lab/HistologyHSI-GB.
• The authors also recommend GLIMPS (https://www.rese-apps.com/software/download/form.html) for the visualization of ENVI images.

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